Mark and Monika at their desks Aurelio setting up a 2D gel
Mark and Monika at their desks
Aurelio setting up a 2D gel
Discussing 2D gel data and possible replication mechanisms with colleagues
standing at front left to right Aurelio Reyes, Ian Holt, Howy Jacobs
After the discussions eating at a local Japanese restaraunt. From left Howy Jacobs (Tampere, Finland), Ian Holt, Heather Lorimer
Three hyb ovens in the hot room, all in use
Above right, three PCR machines, each with three separately
controlled chambers
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Below left to right: The 2D gel group at the Holt lab Christmas dinner December 2003. Ming Yao Yang, Takehiro Yasukama, Heather Lorimer, Aurelio Reyes, Mark Bowmaker. It's been such a pleasure to work with these guys!
Some noteworthy 2D gel/ mtDNA replication papers from the lab:
Bowmaker, M., M. Y. Yang, T. Yasukawa, A. Reyes, H. T. Jacobs, J.A. Huberman, and I. J. Holt. 2003 "Mammalian mitochondrial DNA replicates bidirectionally from an initiation zone" J. Biol. Chem. 278(51):5091-5096.
Yang, M. Y., M. Bowmaker, A. Reyes, L. Vergani, P. Angeli, E. Gringeri, H. T. Jacobs and I. J. Holt. 2002. " Biased incorporation of ribonucleotides on the mitochondiral L-Strand accounts for apparent strand-asymmetric DNA replication. Cell 111: 495-505
Holt, I. J., H. E. Lorimer, H. T. Jacobs. 2000. Coupled leading- and
lagging-strand synthesis of mammalian mitochondrial DNA. Cell 100:515-524.